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Validation of the SolerisĀ® Enterobacteriaceae Method for Detection of Enterobacteriaceae in Dried Cannabis Flower: AOAC Performance Tested MethodSM 121901.
- Source :
-
Journal of AOAC International . Nov/Dec2022, Vol. 105 Issue 6, p1671-1676. 6p. 2 Charts. - Publication Year :
- 2022
-
Abstract
- Background: The Soleris® Enterobacteriaceae vial is a growth-based, automated method for detection of bacteria of the family Enterobacteriaceae in foods and other sample types including nutraceuticals and cosmetics. The Soleris method is used in a "dilute-to-specification" or threshold manner, in which a result is scored as positive or negative around a predetermined cutoff (in CFU/g) established by the dilution and volume of sample homogenate tested. The Soleris method was granted AOAC Performance Tested MethodSM (PTM) status for select foods after successful completion of a validation study (PTM 121901). Objective: The objective of this study was to validate the method for the detection of Enterobacteriaceae in dried cannabis flower [>0.3% delta-9-tetrahydrocannabinol (THC)]. Methods: The matrix study included comparison of Soleris method presumptive results to confirmation from the Soleris vials, and comparison of the Soleris confirmed results to those of the ISO 21528-2:2017 colony count method. Test materials at four different levels of contamination ranging from 7.8 to 3500 CFU/g were tested at three dilutions, corresponding to test thresholds. Results: Probability of detection analysis at P<0.05 showed there were no significant differences between Soleris presumptive and confirmed results, and no significant differences between Soleris confirmed and ISO 21528-2:2017 results. Conclusion: The results provided evidence that the Soleris Enterobacteriaceae test is an accurate method for detection of Enterobacteriaceae in dried cannabis flower. Highlights: The Soleris Enterobacteriaceae method provides cannabis industry QC personnel with an effective method for analysis of dried cannabis flower and produces results in 20-24 h. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10603271
- Volume :
- 105
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of AOAC International
- Publication Type :
- Academic Journal
- Accession number :
- 160194148
- Full Text :
- https://doi.org/10.1093/jaoacint/qsac063