Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway.

1,5-Pentanediol (1,5-PDO) is a high value-added chemical which is widely used as a monomer in the polymer industry. There are no natural organisms that could directly produce 1,5-PDO from renewable carbon sources. In this study, we report metabolic engineering of Escherichia coli for high-level production of 1,5-PDO from glucose via a cadaverine-derived pathway. In the newly proposed pathway, cadaverine can be converted to 1,5-PDO via 5-hydroxyvalerate (5-HV) by introducing only one heterologous enzyme in E. coli. Different endogenous genes of E. coli were screened and heterologous carboxylic acid reductase genes were tested to build a functional pathway. Compared to the previously reported pathways, the engineered cadaverine-based pathway has a higher theoretical yield (0.70 mol/mol glucose) and higher catalytic efficiency. By further combining strategies of pathway engineering and process engineering, we constructed an engineered E. coli strain that could produce 2.62 g/L 1,5-PDO in shake-flask and 9.25 g/L 1,5-PDO with a yield of 0.28 mol/mol glucose in fed-batch fermentation. The proposed new pathway and engineering strategies reported here should be useful for developing biological routes to produce 1,5-PDO for real application. • A cadaverine-based 1,5-PDO synthesis pathway was proposed for the first time. • Strategies to increase driving forces were adopted to increase the pathway efficiency. • Μetabolic engineering and process engineering were combined to increase strain performance. • An engineered strain can directly utilize glucose to produce 1,5-PDO with a titer of 9.25 g/L and a yield of 0.28 mol/mol. [ABSTRACT FROM AUTHOR]