Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics.

The envenomation from the Bothrops genus is characterized by systemic and local effects caused by the main toxin families in the venom. In Bothrops pubescens venom we were able to identify 89 protein groups belonging to 13 toxin families with the bottom-up proteomics approach and 40 unique proteoforms belonging to 6 toxin families with the top-down proteomics approach. We also identified multi-proteoform complexes of dimeric L-amino acid oxidase using native top-down mass spectrometry. [Display omitted] • Bothrops pubescens is a venomous snake endemic of Pampa Biome in south Brazil. • B. pubescens venom has 14 different toxins families. • B. pubescens venom has 89 different proteins identified by bottom-up proteomics. • We were able to identify 40 proteoforms in B. pubescens venom with the Top-Down proteomics approach. • B. pubescens venom is a classical Bothrops venom. [ABSTRACT FROM AUTHOR]