MYT1L promotes the migration and invasion of glioma cells through activation of Notch signaling pathway.

MYT1L gene has been associated with various brain diseases as it affects many neuronal and biological processes of nerve cells. However, the role of MYT1L in glioma cell migration and invasion and its mechanism of action are still unclear. In this study, MYT1L was successfully silenced in U87 and A172 cells, and overexpressed in U251 cells using lentiviral vectors. The expression levels of MYT1L in glioma cells were assessed by real-time PCR and Western blot analysis. The effect of MYT1L on migration and invasion was examined by a Transwell assay. We found that MYT1L enhanced cell migration and invasion whereas knockdown of MYT1L suppressed migration and invasion in glioma cells. We also demonstrated that MYT1L activated the Notch signaling pathway, and that treatment with the Notch inhibitor DAPT inhibited the migration and invasion of glioma cells. These results suggest that MYT1L may be considered a useful and potential target in the treatment of glioma. [ABSTRACT FROM AUTHOR]