Influence of Low-Level Laser Irradiation on Periodontal Ligament Stem Cell Proliferation and Migration in Diabetic Models (Invitro Study).

Background: Periodontal disease is a chronic inflammatory disease caused by periodontal bacteria. Recently, periodontal phototherapy, treatment using various types of lasers, has attracted attention. Purpose: This in vitro study was aimed to compare and evaluate the effect of lasers with different wave lengths on proliferation and differentiation of periodontal ligament stem cells to stimulate periodontal reattachment. Materials and Methods: A randomized and controlled study design was used to evaluate the effect of low-level laser therapy (LLLT) delivered at different doses over dental fibroblast cell culture in high glucose medium. Results: Comparison between MTT Assay results for the normal group under wavelength 635 and 980 under laser energy 0.5, 1, and 2 and after 24, 48, 72, and 96 hours. All results were significant except the results after 24 hours in MTT 635 assay, Conclusion: The response of cell viability was significantly higher for non-diabetic cases in comparison to diabetic ones for both 635 & 980 nm lasers with different doses and at different time points. Our results also suggested that the cell viability was dosed dependent. [ABSTRACT FROM AUTHOR]