Chitosan-Salicylic acid and Zinc sulphate nano-formulations defend against yellow rust in wheat by activating pathogenesis-related genes and enzymes.

Stripe rust instigated by Puccinia striiformis f. sp. tritici causes major yield loss in wheat. In this study, disease resistance was induced in wheat by pre-activation of pathogenesis related (PR) genes using two different nano-formulations (NFs) i.e. Chitosan- Salicylic acid (SA) NFs (CH-NFs) and Zinc sulphate NFs (Zn-NFs). These NFs were synthesized using green approach and were characterized using various techniques. Both NFs effectively controlled stripe rust in wheat genotypes (WH 711 and WH 1123) by significantly increasing activities of phenylalanine ammonia lyase, tyrosine ammonia lyase and polyphenol oxidase enzymes when compared with disease free-control and diseased plants. Total soluble sugar (TSS) level was highest in CH–NF treated plants. TSS was also relatively higher in diseased plants than disease free-control as well as Zn–NF treated plants. Both CH-NFs and Zn-NFs induced the expression of PR genes. In CH–NF treated plants, the relative expression of PR genes was higher on the 3rd day after spraying (DAS) of NFs as compared to diseased and Zn–NF treated plants in both the genotypes. While in case of Zn–NF treated plants, relative expression of PR genes was higher on 5th DAS as compared to diseased and disease free-control plants. Early rise in expression of PR genes due to NF treatments was responsible for disease resistance in both the wheat genotypes as evidenced by a lower average coefficient of infection. These NFs can be synthesized easily with low cost input, are eco-friendly and can be effectively used against yellow rust as well as other wheat diseases. [Display omitted] • Chitosan-Salicylic acid Nano-formulation (CH–NF) induced rust resistance in wheat. • Zinc sulphate NF (Zn–NF) was also able to reduce stripe rust incidence. • Disease score was negligible in CH–NF and low in Zn–NF treated plants. • Activities of resistance enzymes were also increased due to NFs application. • Pre-activation is evident from increased expression of pathogenesis related genes. [ABSTRACT FROM AUTHOR]