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Control of ribosomal RNA synthesis by hematopoietic transcription factors.
- Source :
-
Molecular Cell . Oct2022, Vol. 82 Issue 20, p3826-3826. 1p. - Publication Year :
- 2022
-
Abstract
- Ribosomal RNAs (rRNAs) are the most abundant cellular RNAs, and their synthesis from rDNA repeats by RNA polymerase I accounts for the bulk of all transcription. Despite substantial variation in rRNA transcription rates across cell types, little is known about cell-type-specific factors that bind rDNA and regulate rRNA transcription to meet tissue-specific needs. Using hematopoiesis as a model system, we mapped about 2,200 ChIP-seq datasets for 250 transcription factors (TFs) and chromatin proteins to human and mouse rDNA and identified robust binding of multiple TF families to canonical TF motifs on rDNA. Using a 47S-FISH-Flow assay developed for nascent rRNA quantification, we demonstrated that targeted degradation of C/EBP alpha (CEBPA), a critical hematopoietic TF with conserved rDNA binding, caused rapid reduction in rRNA transcription due to reduced RNA Pol I occupancy. Our work identifies numerous potential rRNA regulators and provides a template for dissection of TF roles in rRNA transcription. [Display omitted] • Multiple cell-type-specific TFs bind canonical motifs on rDNA • The hematopoietic TF CEBPA binds to active rDNA alleles at a conserved site • CEBPA promotes RNA Pol I occupancy and rRNA transcription in myeloid progenitors • We present "47S-FISH-Flow," a sensitive assay to quantify nascent rRNA Antony et al. mapped existing ChIP-seq datasets to human and mouse rDNA and identified binding profiles of cell-type-specific transcription factors (TFs). The myeloid TF CEBPA bound rDNA at a conserved motif, and its degradation reduced rDNA occupancy of the RNA Pol I-RRN3 complex and nascent 47S rRNA transcription. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10972765
- Volume :
- 82
- Issue :
- 20
- Database :
- Academic Search Index
- Journal :
- Molecular Cell
- Publication Type :
- Academic Journal
- Accession number :
- 159858017
- Full Text :
- https://doi.org/10.1016/j.molcel.2022.08.027