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A photoelectrochemical immunosensor based on magnetic all-solid-state Z-scheme heterojunction for SARS-CoV-2 nucleocapsid protein detection.
- Source :
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Sensors & Actuators B: Chemical . Jan2023, Vol. 374, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
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Abstract
- Rapid, convenient and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is urgently needed to timely diagnosis of coronavirus pandemic (COVID-19) and control of the epidemic. In this study, a signal-off photoelectrochemical (PEC) immunosensor was constructed for SARS-CoV-2 nucleocapsid (N) protein detection based on a magnetic all-solid-state Z-scheme heterojunction (Fe 3 O 4 @SiO 2 @TiO 2 @CdS/Au, FSTCA). Integrating the advantages of magnetic materials and all-solid-state Z-scheme heterostructures, FSTCA was implemented to ligate the capture antibody to form magnetic capture probe (FSTCA/Ab 1). It can simplify the separation and washing process to improve reproducibility and stability, while allowing immune recognition to be performed in the liquid phase instead of the traditional solid-liquid interface to improve anti-interference. Besides, the heterojunction inhibited the recombination of photogenerated electron/hole (e-/h+) and promoted the light absorption to provide superior photoelectric substrate signal. The mechanism of photogenerated e−/h+ transfer of FSTCA were investigated by the electron spin resonance (ESR) spectroscopy. SiO 2 spheres loaded with Au NPs utilized as an efficient signal quencher. The steric hindrance effect of SiO 2 @Au labeled detection antibodies (SiO 2 @Au-Ab 2) conjugates significantly diminished light absorption and hindered the transfer of photogenerated electrons, further amplifying the signal change value. Based on the above merits, the elaborated immunosensor had a wide linear range of 10 pg mL−1–100 ng mL−1 and a low detection limit down to 2.9 pg mL−1 (S/N = 3). The fabricated PEC immunosensor demonstrated strong anti-interference, easy operation, and high sensitivity, showing enormous potential in clinical diagnosis of SARS-CoV-2. [Display omitted] • A PEC immunosensor was developed based on FSTCA for detecting SARS-CoV-2N protein. • FSTCA owns strong magnetic response and exceptional PEC performance • Immune recognition was performed in liquid phase and process was simplified. • The mechanism of photogenerated e− /h+ transfer of FSTCA were investigated by the ESR. • The immunosenor possessed a low detection limit of 2.9 pg mL−1. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 374
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 159857785
- Full Text :
- https://doi.org/10.1016/j.snb.2022.132800