Anti-Methicillin-Resistant S. aureus Activity of Fruiting Body and Mycelial Culture Extracts of Xylaria longipes Nitschke (Ascomycota).

Resistance to penicillin by Staphylococcus aureus gave rise to methicillin-resistant S. aureus (MRSA) and the emergence of vancomycin-resistant S. aureus (VRSA) that was reported later. Finding an alternative antimicrobial in the treatment of Staphylococcal infections is the need of the hour. In this context, secondary metabolites of both the fruiting body and mycelia of wood-rotting fungi Xylaria longipes were evaluated for anti-MRSA activity. Thin layer chromatographic (TLC) separation and bioautography of the acetone extract revealed a strong anti-MRSA activity at Rf = 0.69 ± 0.28. The bioactive anti-MRSA compound was partially characterized by Fourier transform infrared (FTIR) spectroscopy and liquid chromatography—tandem mass spectrometry (LC-MS/MS). The analysis suggested anti-MRSA activity could be due to integric acid, eremoxylarin C, or a related compound. [ABSTRACT FROM AUTHOR]