牙髓再生组织工程中的细胞共培养体系.

OBJECTIVE: To summarize research progress on forming dental pulp-like tissue from dental pulp stem cells via a co-culture system, as well as provide ideas for stem cell therapy for dental pulp tissue regeneration. METHODS: The first author searched PubMed, CNKI, and Wanfang databases for relevant literature published before May 2022. The English search terms were “dental pulp regeneration, dental pulp stem cell, co-culture, tissue engineering, signaling pathway”, and the Chinese search terms were “dental pulp regeneration, dental pulp stem cells, co-culture, tissue engineering, scaffold, signaling pathway”. Totally 67 articles meeting the criteria were summarized and described. RESULTS AND CONCLUSION: (1) Different combinations of cells are used in co-culture systems to form the main body of the cell and the combined body. (2) Direct co-culture and indirect co-culture are the two types of combination modes at present. Through the intervention of biological scaffold materials and a change in culture conditions, the co-culture outcome can be divided into two-dimensional regenerated tissues and three-dimensional regenerated tissues. (3) Direct co-culture creates an extracellular matrix and microenvironment that facilitates stem cell proliferation and differentiation through intercellular contact. (4) Indirect co-culture addresses the problem of insufficient sources of combined cells and immune rejection between individuals. The biological factors produced by the combined cells can be more easily made into clinical drugs and biological scaffold materials. (5) Though the co-culture system has superior performance in angiogenesis, there are few reports on other structures, and the regeneration of tissue from the co-culture outcome is uncertain. Furthermore, there are limitations to applying newly generated tissue generated by the co-culture system to clinical practice due to tooth anatomical constraints and rejection mechanisms. [ABSTRACT FROM AUTHOR]