IL-6 抗体对银屑病小鼠的治疗作用及其对外周血辅助性T 细胞 和树突状细胞影响的研究.

Objective: To study the therapeutic effect of IL-6 antibody on psoriatic mice, and to explore the effect of IL-6 antibody treatment on peripheral blood helper T cells and dendritic cells of psoriatic mice. Methods:Thirty 6-8 week old SPF grade BALB/c mice were divided into Normal group, Model group and IL-6 group according to the random number table method. The Model group and IL-6 group were used to establish psoriasis mouse models by applying imiquimod. Mice in the IL-6 group were treated by subcutaneous injection of IL-6 antibody in the dorsal alopecia. The psoriasis area and severity index (PASI), epidermal thickness and number of blood vessels in the skin lesions of the three groups of mice were compared, serum TNF-α, IL-17 and IL-23 levels, peripheral blood dendritic cells, Th17 cells and Th22 cells ratios and IL-6, IL-21 and STAT3 protein expressions in skin lesions. Results: After IL-6 antibody treatment, PASI, epidermal thickness and blood vessel number, serum TNF-α, IL-17 and IL-23 contents, peripheral blood dendritic cells and Th17 cells of mice in Model group and IL-6 group were compared. The proportions of IL-6, IL-21 and STAT3 in skin lesions were significantly higher than those in the Normal group (P<0.05); compared with the Model group, the PASI in the skin lesions of the IL-6 group mice, epidermal thickness and blood vessel number, serum TNF-α, IL-17 and IL-23 levels, peripheral blood dendritic cells, Th17 cells and Th22 cell ratios, and IL-6, IL-21 and STAT3 protein expressions in skin lesions were significantly decreased (P<0.05). Conclusion: IL-6 antibody has a good therapeutic effect on the psoriasis mouse model, and its mechanism may be related to the influence of Th17/Th22 and dendritic cell levels by inhibiting the IL-6/STAT3 pathway. [ABSTRACT FROM AUTHOR]