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miR-2188-5p promotes GCRV replication by the targeted degradation of klf2a in Ctenopharyngodon idellus.
- Source :
-
Developmental & Comparative Immunology . Jan2023, Vol. 138, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
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Abstract
- Studies on host immunity evasion by aquatic viruses have largely focused on coding genes. There is accumulating evidence for the important biological functions of non-coding miRNAs in virus–host interactions. The regulatory functions of non-coding miRNAs in fish reovirus–host interactions remain unknown. Here, miR-2188-5p in grass carp (Ctenopharyngodon idellus), a miRNA specific to teleosts, was predicted to target the 3′ UTR of the transcription factor klf2a. A correlation analysis and dual-luciferase reporter assay revealed that miR-2188-5p could induce the degradation of klf2a. The expression of miR-2188-5p induced the degradation of klf2a in a dose-dependent manner, suppressing the type I interferon response and promoting grass carp reovirus (GCRV) replication. As determined by a co-expression analysis , klf2a inhibited viral infection when miR-2188-5p was overexpressed. The targeted degradation of klf2a by miR-2188-5p could inhibit the type I interferon response and promote the replication of GCRV; however, this targeted degradation ability was insufficient to fully inhibit GCRV infection. These results provide novel insights into the regulatory effects and biological functions of non-coding miRNAs in fish–virus interactions. • Grass carp miR-2188-5p targets the degradation of klf2a. • miR-2188-5p targets klf2a , suppresses IFN1 and promotes GCRV replication. • klf2a expression offsets the induced expression of the GCRV genome by miR-2188-5p. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0145305X
- Volume :
- 138
- Database :
- Academic Search Index
- Journal :
- Developmental & Comparative Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 159569074
- Full Text :
- https://doi.org/10.1016/j.dci.2022.104516