Study on Tissue Culture and Rapid Propagation of Tilia amurensis.

To explore the establishment of a tissue culture and rapid propagation system of Tilia amurensis, the effects of basic medium and concentrations and ratios of plant growth reaulatorr on tissue culture and rapid propagation of T amurensis were studied. The results showed that 1/2MS medium was the most suitable proliferation medium, and the proliferation coefficient coulO reach 13. 5 after adding 0. 05 mg/L 6-BA and 0. 03 mg/L IBA; MS medium was the most suitable medium for strong plantets and rooting, and the best medium for rUong plantlets was MS +0.1 mg/L 6-BA +0.1 mg/L IBA +0. 03 mg/L GA3, with which the average plantet height reached 5.15 cm; and the best rooting medium was MS + 1.0 mg/L6-BA +0. 05 mg/L NAA, with which the rooûng rate was 93. 3% and the number of roots was 5.7 roots. [ABSTRACT FROM AUTHOR]