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Subpopulations of CD4+ cells in lpr/lpr mice: differences in expression of T cell receptor/CD 3 complex and proliferative responses.
- Source :
-
Clinical & Experimental Immunology . Jul1990, Vol. 81 Issue 1, p90-96. 7p. - Publication Year :
- 1990
-
Abstract
- CD4+ cells from autoimmune-prone C57BL/6 lpr/lpr mice contain two subpopulations. B220- CD4+ and B220+CD4+ cells. Highly purified B220- CD4+ cells from C57BL/6 +/+ and lpr/lpr mice were examined by comparing functional characteristics and expression of cell surface antigens and T cell receptor (TcR)/CD3 complex. Both lpr B220+CD4+ and B220+CD4- CD8- cells, most of which were PgP-1 positive, expressed TcR/CD3 complex on the cell surface at lower level as compared with B220- CD4+ cells of age-matched normal mice. In addition, the B220- CD4+ cells were heterogeneous on the basis of surface expression of PgP-1 and CD3 antigens. Normal levels of TcR Cα-. Cβ- and Vβ8-specific mRNA were found in the B220-CD4+ cells and B220+CD4+ cells as compared with normal B220+CD4+ cells, while Vβ8-specific mRNA was preferentially expressed only by B220+CD4--CD8- cells. Either B220+CD4+ cells and B220+CD4- CD8- cells failed to respond to anti-CD3 monoclonal antibody (MoAb) as assessed by proliferative responses and production of interleukin-2 (IL-2). However, appreciable levels of reactivity to anti-CD3 MoAb were detected in the B220- CD4+ cells, although the responsiveness of this subset to such stimuli were reduced, compared with those of normal control. These results indicate that the B220- CD4+ cells in lpr mice are phenotypically and functionally distinct from normal B220-CD4+ cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 81
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15928521