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Rational Design of Porous Poly(ethylene glycol) Films as a Matrix for ssDNA Immobilization and Hybridization.
- Source :
-
Bioengineering (Basel) . Sep2022, Vol. 9 Issue 9, p414-414. 16p. - Publication Year :
- 2022
-
Abstract
- Poly(ethylene glycol) (PEG) films, fabricated by thermally induced crosslinking of amine- and epoxy-terminated four-arm STAR-PEG precursors, were used as porous and bioinert matrix for single-stranded DNA (ssDNA) immobilization and hybridization. The immobilization relied on the reaction between the amine groups in the films and N-hydroxy succinimide (NHS) ester groups of the NHS-ester-decorated ssDNA. Whereas the amount of reactive amine groups in the films with the standard 1:1 composition of the precursors turned out to be too low for efficient immobilization, it could be increased noticeably using an excess (2:1) concentration of the amine-terminated precursor. The respective films retained the bioinertness of the 1:1 prototype and could be successfully decorated with probe ssDNA, resulting in porous, 3D PEG-ssDNA sensing assemblies. These assemblies exhibited high selectivity with respect to the target ssDNA strands, with a hybridization efficiency of 78–89% for the matching sequences and full inertness for non-complementary strands. The respective strategy can be applied to the fabrication of DNA microarrays and DNA sensors. As a suitable transduction technique, requiring no ssDNA labeling and showing high sensitivity in the PEG-ssDNA case, electrochemical impedance spectroscopy is suggested. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SINGLE-stranded DNA
*DNA microarrays
*IMPEDANCE spectroscopy
Subjects
Details
- Language :
- English
- ISSN :
- 23065354
- Volume :
- 9
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Bioengineering (Basel)
- Publication Type :
- Academic Journal
- Accession number :
- 159274925
- Full Text :
- https://doi.org/10.3390/bioengineering9090414