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Design, synthesis and evaluation of 15N- and 13C-labeled molecular probes as hyperpolarized nitric oxide sensors.

Authors :
Park, Hyejin
Eriksson, Shannon
Warren, Warren S.
Wang, Qiu
Source :
Bioorganic & Medicinal Chemistry. Oct2022, Vol. 72, pN.PAG-N.PAG. 1p.
Publication Year :
2022

Abstract

We report the design and development of novel 15N-and 13C-labeled NO-sensing probes for hyperpolarized nuclear magnetic resonance (HP-NMR) studies. These probes undergo selective and rapid reaction with NO to generate the AZO-products in situ that can be monitored with distinguishable NMR signals as a read-out. This study also provides direct comparison of the 15N and 13C nuclei performances in hyperpolarized reaction-based probes. [Display omitted] [Display omitted] Nitric oxide (NO) is an important signaling molecule involved in a wide range of biological processes. Development of non-invasive, real-time detection of NO is greatly desired yet remains challenging. Here we report the design and development of novel 15N- and 13C-labeled NO-sensing probes for hyperpolarized nuclear magnetic resonance (HP-NMR) studies. These probes undergo selective and rapid reaction with NO to generate in situ AZO-products that can be monitored with distinguishable NMR signals as a read-out. This study also allows for a direct comparison of the 15N and 13C nuclei performances in hyperpolarized reaction-based probes. The simple and general SABRE-SHEATH hyperpolarization method works on the 15N- and 13C-NO-sensing probes. Measured long spin–lattice relaxation (T 1) values, especially for 15N-NO probes, will allow for real-time reaction-based imaging of NO. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09680896
Volume :
72
Database :
Academic Search Index
Journal :
Bioorganic & Medicinal Chemistry
Publication Type :
Academic Journal
Accession number :
159168430
Full Text :
https://doi.org/10.1016/j.bmc.2022.116969