Development of a rat model for type 2 diabetes mellitus peri‐implantitis: A preliminary study.

Objective: To develop an in vivo model to simulate the complex internal environment of diabetic peri‐implantitis (T2DM‐PI) model for a better understanding of peri‐implantitis in type 2 diabetic patients. Materials and methods: Maxillary first molars were extracted in Sprague‐Dawley (SD) rats, and customized cone‐shaped titanium implants were installed in the extraction sites. Thereafter, implants were uncovered and customized abutments were screwed into implants. A high‐fat diet and a low‐dose injection of streptozotocin were utilized to induce T2DM. Finally, LPS was locally injected in implant sulcus to induce peri‐implantitis. Results: In the present study, T2DM‐PI model has been successfully established. Imaging analysis revealed that abundant inflammatory cells infiltrated in the soft tissue in T2DM‐PI group with concomitant excessive secretion of inflammatory cytokines. Moreover, higher expression of MMP and increased number of osteoclasts led to collagen disintegration and bone resorption in T2DM‐PI group. Conclusions: These results describe a novel rat model which stimulate T2DM‐PI in vivo, characterized by overwhelming inflammatory response and bone resorption. This model has a potential to be used for investigation of initiation, progression and interventional therapy of T2DM‐PI. [ABSTRACT FROM AUTHOR]