蔗渣纤维素预处理及纤维素酶混合发酵研究.

The experiment was to study the pretreatment method of bagasse cellulose, optimize the composition of cellulase system and increase the amount of enzyme production. Bagasse cellulose was pretreated by microwave, phosphoric acid and steam explosion. The results showed that cellulase production and hemicellulase activity of Fusarium oxychlamycline HML278 were increased, the enzyme activity of filter paper was increased by 1.02 times than that of untreated material, and the enzymatic hydrolysis of glucose was increased by 100% compared with that of untreated bagasse. Aspergillus oryzae HML366 could produce β-glucosidase with high transferring-activity to synthesize gentiose. Gentiose could induce cellulase production from Fusarium chlamychii HML278. Fusarium oxychlamycline HML278 and Aspergillus oryzae HML366 had good compatibility, and the enzyme activity of filter paper was increased by 83.62% compared with Fusarium oxychlamycline HML278 and 93.09% compared with Aspergillus oryzae HML366. The study indicates that the mixed culture of Aspergillus oryzae HML366 and Fusarium oxychlamyclum HML278 is better than that of single strain. [ABSTRACT FROM AUTHOR]