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ATG4B hinders porcine epidemic diarrhea virus replication through interacting with TRAF3 and activating type-I IFN signaling.
- Source :
-
Veterinary Microbiology . Oct2022, Vol. 273, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- Autophagy-related 4B (ATG4B) is found to exert a vital function in viral replication, although the mechanism through which ATG4B activates type-I IFN signaling to hinder viral replication remains to be explained, so far. The current work revealed that ATG4B was downregulated in porcine epidemic diarrhea virus (PEDV)-infected LLC-PK1 cells. In addition, ATG4B overexpression inhibited PEDV replication in both Vero cells and LLC-PK1 cells. On the contrary, ATG4B knockdown facilitated PEDV replication. Moreover, ATG4B was observed to hinder PEDV replication by activating type-I IFN signaling. Further detailed analysis revealed that the ATG4B protein targeted and upregulated the TRAF3 protein to induce IFN expression via the TRAF3-pTBK1-pIRF3 pathway. The above data revealed a novel mechanism underlying the ATG4B-mediated viral restriction, thereby providing novel possibilities for preventing and controlling PEDV. • ATG4B was downregulated in porcine epidemic diarrhea virus (PEDV)-infected LLC-PK1 cells. • ATG4B overexpression inhibited PEDV proliferation in both Vero cells and LLC-PK1 cells. • ATG4B knockdown promoted PEDV replication. • ATG4B hindered PEDV replication by activating type-I IFN signaling via the TRAF3-pTBK1-pIRF3 pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PORCINE epidemic diarrhea virus
*VIRAL replication
Subjects
Details
- Language :
- English
- ISSN :
- 03781135
- Volume :
- 273
- Database :
- Academic Search Index
- Journal :
- Veterinary Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 158931159
- Full Text :
- https://doi.org/10.1016/j.vetmic.2022.109544