呼吸道合胞病毒毛细支气管炎婴幼患儿外周血 miR-155 与 PDCD4 表达的相关性研究.

Abstract: Objective To explore the relationship between the expression of miR-155 and programmed cell death 4 (PDCD4) in peripheral blood of infants with respiratory syncytial virus (RSV) bronchiolitis. Methods A total of 106 infants and young children with RSV bronchiolitis accepted by our hospital were collected as the research group. According to the severity of the disease, patients were separated into the mild group (n=36), the moderate group (n=41), and the severe group (n=29). Sixty-five infants who underwent physical examination in the child care clinic of our hospital were selected as the control group (n=55). The serum level of miR-155 in peripheral blood of infants and young children was measured by real-time quantitative PCR (qPCR) method. Levels of PDCD4, high sensitivity C-reactive protein (hs-CRP), interleukin (IL)-6, IL-10 and tumor necrosis factor (TNF)-α were measured by enzyme-linked immunosorbent assay (ELISA). The relationship between serum miR-155 and PDCD4 and their correlation with inflammatory factors in infants with RSV bronchiolitis were analyzed by Pearson correlation. Results Compared with the control group, the serum level of miR-155 was significantly increased in the study group, and the level of PDCD4 was significantly decreased (P＜0.05). In addition, the serum miR-155 level increased with the aggravation of the severity of RSV bronchiolitis, and the PDCD4 level reduced significantly (P＜ 0.05). Compared with the control group, serum levels of inflammatory factors hs-CRP, IL-6, IL-10 and TNF-α increased significantly in the study group (P＜0.05). Pearson correlation analysis showed that miR-155 and PDCD4 were negatively correlated (r=-0.790, P＜0.01), miR-155 was positively correlated with hs-CRP, IL-6, IL-10, and TNF-α (P＜0.01), and PDCD4 was negatively correlated with hs-CRP, IL-6, IL-10, and TNF-α (P＜0.01). Conclusion RSV bronchiolitis in infants and young children has abnormal expression of serum levels of miR-155 and PDCD4, and the two levels are negatively correlated. Serum levels of miR-155 and PDCD4 are tightly related to inflammatory factors. [ABSTRACT FROM AUTHOR]