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Mutagenesis analysis of GMN motif in Arabidopsis thaliana Mg2+ transporter MRS2-1.

Authors :
Xiaoyu Yang
Kobayashi, Natsuko I.
Yoshiki Hayashi
Koichi Ito
Yoshitaka Moriwaki
Tohru Terada
Kentaro Shimizu
Motoyuki Hattori
Ren Iwata
Hisashi Suzuki
Nakanishi, Tomoko M.
Keitaro Tanoi
Source :
Bioscience, Biotechnology & Biochemistry. Jul2022, Vol. 86 Issue 7, p870-874. 5p.
Publication Year :
2022

Abstract

Magnesium is an important nutrient for plants, but much is still unknown about plant Mg2+ transporters. Combining with the structural prediction of AlphaFold2, we used mutagenesis and 28Mg uptake assay to study the highly conserved "GMN" motif of Arabidopsis thaliana MRS2-1 (AtMRS2-1) transporter. We demonstrated that the glycine and methionine in GMN motif are essential for AtMRS2-1 to transport Mg2+. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09168451
Volume :
86
Issue :
7
Database :
Academic Search Index
Journal :
Bioscience, Biotechnology & Biochemistry
Publication Type :
Academic Journal
Accession number :
158721000
Full Text :
https://doi.org/10.1093/bbb/zbac064