Ole e 2 and Ole e 10: new clinical aspects and genetic restrictions in olive pollen allergy.

The clinical characteristics in olive pollen allergy are dependent on the antigenic load, the allergens profile, and the genetic restrictions. Our objective was to determine specific response pattern in Ole e 2 and Ole e 10 sensitization at those levels.We studied 146 patients with seasonal rhinitis and/or asthma and positive prick test toOlea europaeapollen. IgE against Ole e 2 and Ole e 10 were detected by skin prick test and ELISA. HLA-DRB1 and HLA-DQB1 loci were typed by polymerase chain reaction sequence-specific primers method.A total of 102 (69.9%) and 79 (54.0%) patients showed significant IgE antibody response against Ole e 2 and Ole e 10, respectively. There was a significant association between Ole e 2 (OR 2.2,P = 0.04) and Ole e 10 reactivities (OR 2.8,P = 0.007) with asthma. In addition, total and specific IgE antibody levels significantly correlated with asthma (P < 0.05). Patients who reacted to both allergens reached the highest asthma risk factor (OR 4.3,P = 0.002). Phenotypic frequency of DR7 (OR 5.4,Pc = 0.003) and DQ2 (OR 3.6,Pc = 0.02) were increased in positive Ole e 2 patients compared with control subjects. DR2(15) phenotypic frequency was significantly increased (OR 5.6,Pc = 0.02) in positive Ole e 10 patients compared with control subjects.Our data suggest an association of Ole e 2 and Ole e 10 with bronchial asthma. Also, we found a genetic control of Ole e 2 and Ole e 10 IgE-specific responses that could be relevant to clinical disease in olive pollen allergy. [ABSTRACT FROM AUTHOR]