Back to Search
Start Over
Letter to the Editor: Speedy Plant Genotyping by SDS-Tolerant Cyclodextrin-PCR.
- Source :
-
Plant & Cell Physiology . Aug2022, Vol. 63 Issue 8, p1025-1028. 4p. - Publication Year :
- 2022
-
Abstract
- Genotyping of Plants by Combining Alkaline/SDS gDNA Extraction and CD-PCR CD-PCR was applied to the genotyping procedure of an I Arabidopsis thaliana i T-DNA tagline mutant (Fig. For both I mot1-1 i (a mutant T-DNA tagline of the molybdate transporter) and I abl2c i (a mutant T-DNA tagline of the anthocyanin-bluing transporter), the wild-type alleles and T-DNA-tagged mutant alleles were successfully identified by migration of allele-specific PCR products (Fig. The introduction of polymerase chain reaction (PCR) has dramatically simplified the gene determination process; however, preparing genomic DNA (gDNA) from test plants is still a time-consuming task. In a PCR that amplifies a 2-kb DNA fragment, -CD made a PCR containing at least 0.06% SDS possible (Supplementary Fig. [Extracted from the article]
Details
- Language :
- English
- ISSN :
- 00320781
- Volume :
- 63
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Plant & Cell Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 158570268
- Full Text :
- https://doi.org/10.1093/pcp/pcac093