Letter to the Editor: Speedy Plant Genotyping by SDS-Tolerant Cyclodextrin-PCR.

Genotyping of Plants by Combining Alkaline/SDS gDNA Extraction and CD-PCR CD-PCR was applied to the genotyping procedure of an I Arabidopsis thaliana i T-DNA tagline mutant (Fig. For both I mot1-1 i (a mutant T-DNA tagline of the molybdate transporter) and I abl2c i (a mutant T-DNA tagline of the anthocyanin-bluing transporter), the wild-type alleles and T-DNA-tagged mutant alleles were successfully identified by migration of allele-specific PCR products (Fig. The introduction of polymerase chain reaction (PCR) has dramatically simplified the gene determination process; however, preparing genomic DNA (gDNA) from test plants is still a time-consuming task. In a PCR that amplifies a 2-kb DNA fragment, -CD made a PCR containing at least 0.06% SDS possible (Supplementary Fig. [Extracted from the article]