Back to Search
Start Over
Protein kinase Cε is dispensable for TCR/CD3-signaling
Protein kinase Cε is dispensable for TCR/CD3-signaling
- Source :
-
Molecular Immunology . Feb2005, Vol. 42 Issue 3, p305-310. 6p. - Publication Year :
- 2005
-
Abstract
- Abstract: PKCε has been strongly linked to cell activation and proliferation in many cell types, including leukemic T-cell lines. In particularly, an essential role of PKCε has been established in the IKK-β/I-κB/NF-κB transactivation cascade. To study the physiological function of PKCε in primary T-cells, we used our newly established PKCε null mice. Unexpectedly, however, we did not reveal any defect in the development and function of CD3+ T-cells. Proliferative responses as well as IL-2 cytokine secretion of PKCε-deficient T-cells induced by allogenic MHC, plate-bound anti-CD3 antibodies (with or without anti-CD28 costimulation), or mitogenic stimuli such as phorbol ester and Ca2+ ionophore were comparable with wild-type controls. Consistently, after CD3/CD28 engagement, deficiency of PKCε did not impair NF-κB transactivation as well as CD25, CD44 and CD69 induction. Thus, PKCε-deficient T-cells had similar physiological thresholds for activation in vitro. This finding suggests that PKCε plays a redundant role in TCR-induced regulation of T-cell proliferation. [Copyright &y& Elsevier]
- Subjects :
- *PROTEIN kinases
*T cells
*INTERLEUKIN-2
*LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 01615890
- Volume :
- 42
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Molecular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15837943
- Full Text :
- https://doi.org/10.1016/j.molimm.2004.07.007