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Biotin deficiency reduces expression of SLC19A3, a potential biotin transporter, in leukocytes from human blood.
- Source :
-
Journal of Nutrition . Jan2005, Vol. 135 Issue 1, p42-47. 6p. 3 Charts, 4 Graphs. - Publication Year :
- 2005
-
Abstract
- In evaluating potential indicators of biotin status, we quantitated the expression of biotin-related genes in leukocytes from human blood of normal subjects before and after inducing marginal biotin deficiency. Biotin deficiency was induced experimentally by feeding an egg-white diet for 28 d. Gene expression was quantitated for the following biotin-related proteins: methylcrotonyl-CoA carboxylase chains A (MCCA) and B (MCCB); propionyl-CoA carboxylase chains A (PCCA) and B (PCCB); pyruvate carboxylase (PC); acetyl-CoA carboxylase isoforms A (ACCA) and B (ACCB); holocarboxylase synthetase (HCS); biotinidase; and 2 potential biotin transporters: sodium-dependent multivitamin transporter (SMVT) and solute carrier family 19 member 3 (SLC19A3). For 7 subjects who successfully completed the study, the abundance of the specific mRNAs was determined by quantitative real-time RT-PCR at d 0 and 28. At d 28, SLC19A3 expression had decreased to 33% of d 0 (P < 0.02 by two-tailed, paired t test). Expression of MCCA, PCCA, PC, ACCA, ACCB, HCS, biotinidase, and SMVT decreased to approximately 80% of d 0 (P < 0.05). Expression of the MCCB and PCCB chains that do not carry the biotin-binding motif did not change significantly; we speculate that expression of the biotin-binding chains of biotin-dependent carboxylases is more responsive to biotin status changes. These data provide evidence that expression of SLC19A3 is a relatively sensitive indicator of marginal biotin deficiency. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00223166
- Volume :
- 135
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 15804949
- Full Text :
- https://doi.org/10.1093/jn/135.1.42