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Stress conditions induced circRNAs profile of extracellular vesicles in brain microvascular endothelial cells.
- Source :
-
Metabolic Brain Disease . Aug2022, Vol. 37 Issue 6, p1977-1987. 11p. - Publication Year :
- 2022
-
Abstract
- Cerebral ischemia causes hypoxic injury and inflammation, and brain microvascular endothelial cells (BMVECs) dysfunction is an initial stage of blood-brain barrier disruption. Endothelial cells secrete extracellular vesicles (EVs) that are involved in intercellular signal transduction. EVs contain a variety of RNAs, proteins, and metabolites. Circular RNA (circRNA) is a member of the non-coding RNA. The expression profile and potential function of circRNAs in BMVECs are unknown. Here, human BMVECs have undergone hypoxia or TNF-α induction, and the changes in circRNAs were measured by RNA sequencing. A total of 70 circRNAs showed differential expression, including 43 previously unrecorded circRNAs and 27 recorded circRNAs. Since astrocyte end-feet encircle endothelial cells, they are considered the main targets of the EVs from BMVEC. The miRNA sequence data and bioinformatics were used to predict the circRNA-miRNA-mRNA networks in astrocytes. The gene ontology (GO) analysis showed the main downstream targets of circRNAs are DNA transcription regulation and protein kinase-related signaling pathways. These results suggest that altering circRNAs may be a potential therapeutic target for cerebral ischemia induced hypoxic injury and inflammation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08857490
- Volume :
- 37
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Metabolic Brain Disease
- Publication Type :
- Academic Journal
- Accession number :
- 157987432
- Full Text :
- https://doi.org/10.1007/s11011-022-01025-1