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A comprehensive molecular approach in fish allergy: Usefulness in daily clinical practice.
- Source :
-
Clinica Chimica Acta . Aug2022, Vol. 533, p104-108. 5p. - Publication Year :
- 2022
-
Abstract
- • Diagnosis of fish may represent an important challenge for the allergists. • There is not a complete cross reactivity among different ß-parvalbumins. • Comprehensive panel of fish allergens is useful for the diagnosis of allergy. Diagnosis of fish may represent an important challenge for the allergists. This study aimed to evaluate the diagnostic sensitivity of an in vitro multiplex assay using a comprehensive panel of fish allergens and the cross-reactivity patterns between different molecular components. 56 subjects with fish allergy were enrolled. All patients underwent specific IgE measurement using the Allergy Explorer-Alex 2™ multiplex assay (Macroarray Diagnostics, Vienna, Austria) The single ß-parvalbumins Clu h 1, Cyp c 1, Gad m 1, Sal s 1, Sco s 1, Thu a 1 and Xyp g 1 scored positive in 75.0%, 67.8%, 62.5%, 80.3%,80.3%, 78.8% and 73,2% patients, respectively. 14.3% scored positive for the α-parvalbumin (Raj c-parvalbumin), and 16.1% for the aldolase + enolase (Gad m 2 + 3) components. 92.8% reacted to at least one ß-parvalbumin and 96.4% to at least one of the allergens tested. Overall sensitivity was higher than that obtained using commercial extracts of cod, salmon and tuna for skin prick test (75.8%) and IgE detection (92.3%). The Alex 2 showed high diagnostic sensitivity and it might be used as an additional assay to investigate the cross-reactivity patterns between different molecular components, looking for potentially safe fish species. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DERMATOPHAGOIDES pteronyssinus
*ALLERGIES
*ENOLASE
*IMMUNOGLOBULIN E
*SKIN tests
Subjects
Details
- Language :
- English
- ISSN :
- 00098981
- Volume :
- 533
- Database :
- Academic Search Index
- Journal :
- Clinica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 157948790
- Full Text :
- https://doi.org/10.1016/j.cca.2022.06.010