Back to Search Start Over

A comprehensive molecular approach in fish allergy: Usefulness in daily clinical practice.

Authors :
Villalta, Danilo
Scala, Enrico
Deleonardi, Gaia
Busa, Moira
Rivolta, Federica
Boni, Elisa
Gabrielli, Giulia
Farioli, Laura
Cappelletti, Camilla
Buzzulini, Francesca
Conte, Mariaelisabetta
Pravettoni, Valerio
Asero, Riccardo
Source :
Clinica Chimica Acta. Aug2022, Vol. 533, p104-108. 5p.
Publication Year :
2022

Abstract

• Diagnosis of fish may represent an important challenge for the allergists. • There is not a complete cross reactivity among different ß-parvalbumins. • Comprehensive panel of fish allergens is useful for the diagnosis of allergy. Diagnosis of fish may represent an important challenge for the allergists. This study aimed to evaluate the diagnostic sensitivity of an in vitro multiplex assay using a comprehensive panel of fish allergens and the cross-reactivity patterns between different molecular components. 56 subjects with fish allergy were enrolled. All patients underwent specific IgE measurement using the Allergy Explorer-Alex 2™ multiplex assay (Macroarray Diagnostics, Vienna, Austria) The single ß-parvalbumins Clu h 1, Cyp c 1, Gad m 1, Sal s 1, Sco s 1, Thu a 1 and Xyp g 1 scored positive in 75.0%, 67.8%, 62.5%, 80.3%,80.3%, 78.8% and 73,2% patients, respectively. 14.3% scored positive for the α-parvalbumin (Raj c-parvalbumin), and 16.1% for the aldolase + enolase (Gad m 2 + 3) components. 92.8% reacted to at least one ß-parvalbumin and 96.4% to at least one of the allergens tested. Overall sensitivity was higher than that obtained using commercial extracts of cod, salmon and tuna for skin prick test (75.8%) and IgE detection (92.3%). The Alex 2 showed high diagnostic sensitivity and it might be used as an additional assay to investigate the cross-reactivity patterns between different molecular components, looking for potentially safe fish species. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00098981
Volume :
533
Database :
Academic Search Index
Journal :
Clinica Chimica Acta
Publication Type :
Academic Journal
Accession number :
157948790
Full Text :
https://doi.org/10.1016/j.cca.2022.06.010