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Systems approaches reveal that ABCB and PIN proteins mediate co-dependent auxin efflux.
- Source :
-
Plant Cell . Jun2022, Vol. 34 Issue 6, p2309-2327. 19p. - Publication Year :
- 2022
-
Abstract
- Members of the B family of membrane-bound ATP-binding cassette (ABC) transporters represent key components of the auxin efflux machinery in plants. Over the last two decades, experimental studies have shown that modifying ATPbinding cassette sub-family B (ABCB) expression affects auxin distribution and plant phenotypes. However, precisely how ABCB proteins transport auxin in conjunction with the more widely studied family of PIN-formed (PIN) auxin efflux transporters is unclear, and studies using heterologous systems have produced conflicting results. Here, we integrate ABCB localization data into a multicellular model of auxin transport in the Arabidopsis thaliana root tip to predict how ABCB-mediated auxin transport impacts organ-scale auxin distribution. We use our model to test five potential ABCB-PIN regulatory interactions, simulating the auxin dynamics for each interaction and quantitatively comparing the predictions with experimental images of the DII-VENUS auxin reporter in wild-type and abcb single and double loss-offunction mutants. Only specific ABCB-PIN regulatory interactions result in predictions that recreate the experimentally observed DII-VENUS distributions and long-distance auxin transport. Our results suggest that ABCBs enable auxin efflux independently of PINs; however, PIN-mediated auxin efflux is predominantly through a co-dependent efflux where colocalized with ABCBs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10404651
- Volume :
- 34
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Plant Cell
- Publication Type :
- Academic Journal
- Accession number :
- 157880234
- Full Text :
- https://doi.org/10.1093/plcell/koac086