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Bovine genital leptospirosis: Evidence of ovarian infection by Leptospira interrogans.

Authors :
dos Santos Pereira, Paulo Victor
Di Azevedo, Maria Isabel Nogueira
dos Santos Baptista Borges, Ana Luiza
Loureiro, Ana Paula
Martins, Gabriel
Carvalho-Costa, Filipe Anibal
Souza-Fabjan, Joanna Maria Gonçalves
Lilenbaum, Walter
Source :
Veterinary Microbiology. Aug2022, Vol. 271, pN.PAG-N.PAG. 1p.
Publication Year :
2022

Abstract

Leptospirosis in ruminants causes reproductive failures leading to important economic losses. This study assessed the occurrence and genetically identified Leptospira spp. in the follicular fluid (FF) of naturally infected live cows. A total of 251 asymptomatic cows from different commercial dairy herds were subjected to ovum -pick up technique for follicular fluid sampling. PCR was performed for Leptospira spp. detection and phylogenetic analysis was later implemented for sequencing. From 251 samples analyzed, 67 (26.7 %) were lipL32 -PCR positive, confirming the presence of leptospiral DNA on FF. Furthermore, it was possible to amplify and sequence nine strains after secY nested-PCR. All of them were identified as L. interrogans, with 100 % of identity with strains belonging to Sejroe serogroup. Our findings reveal a high occurrence of infection of Leptospira in the ovarium of asymptomatic cows, highlighting the importance of considering the silent leptospirosis syndrome when screening animals for assisted reproductive biotechniques. • Bovine genital leptospirosis may decrease the quality of the oocyte. • Leptospiral DNA is present in follicular fluid samples of asymptomatic cows. • Molecular analysis detected strains of Leptospira interrogans from serogroup Sejroe. • Genital samples are essential for detection of infection in reproductive tract. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03781135
Volume :
271
Database :
Academic Search Index
Journal :
Veterinary Microbiology
Publication Type :
Academic Journal
Accession number :
157838989
Full Text :
https://doi.org/10.1016/j.vetmic.2022.109489