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Bovine genital leptospirosis: Evidence of ovarian infection by Leptospira interrogans.
- Source :
-
Veterinary Microbiology . Aug2022, Vol. 271, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- Leptospirosis in ruminants causes reproductive failures leading to important economic losses. This study assessed the occurrence and genetically identified Leptospira spp. in the follicular fluid (FF) of naturally infected live cows. A total of 251 asymptomatic cows from different commercial dairy herds were subjected to ovum -pick up technique for follicular fluid sampling. PCR was performed for Leptospira spp. detection and phylogenetic analysis was later implemented for sequencing. From 251 samples analyzed, 67 (26.7 %) were lipL32 -PCR positive, confirming the presence of leptospiral DNA on FF. Furthermore, it was possible to amplify and sequence nine strains after secY nested-PCR. All of them were identified as L. interrogans, with 100 % of identity with strains belonging to Sejroe serogroup. Our findings reveal a high occurrence of infection of Leptospira in the ovarium of asymptomatic cows, highlighting the importance of considering the silent leptospirosis syndrome when screening animals for assisted reproductive biotechniques. • Bovine genital leptospirosis may decrease the quality of the oocyte. • Leptospiral DNA is present in follicular fluid samples of asymptomatic cows. • Molecular analysis detected strains of Leptospira interrogans from serogroup Sejroe. • Genital samples are essential for detection of infection in reproductive tract. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03781135
- Volume :
- 271
- Database :
- Academic Search Index
- Journal :
- Veterinary Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 157838989
- Full Text :
- https://doi.org/10.1016/j.vetmic.2022.109489