Development of CryoVR, a virtual reality training system for hands‐on cryoEM operations.

Cryogenic electron microscopy (cryoEM) has emerged as a revolutionary method for solving high‐resolution structures and studying the dynamics of macromolecular complexes and viruses in near‐native states. However, the availability of the equipment, and the time and cost needed for training, severely limit the opportunities for training. To solve these problems, a virtual reality‐based training system, CryoVR, has been developed to prepare trainees before operating real‐world cryoEM equipment. This paper describes the design and assessment of CryoVR (available at https://www.purdue.edu/cryoVR), which helps users learn cryoEM experimental procedures in a virtual environment, allowing immersive training with step‐by‐step tutorials with vivid visual, audio and text guidance. Implemented as a training step before a novice user interacts with the expensive real‐world cryoEM equipment, CryoVR can help users to become familiar with hands‐on operational procedures through multiple training modules and earning certificates after passing the built‐in Exam mode. Qualitative evaluation and feedback of CryoVR from users with various levels of cryoEM experience indicate the substantial value of CryoVR as a tool for a comprehensive cryoEM procedural training. [ABSTRACT FROM AUTHOR]