基因差异表达谱及WGCNA 构建骨质疏松症miRNAmRNA 调控网络.

Objective To construct and analyze the miRNA-mRNA regulatory network related to osteoporosis via gene differentially expressed profile and WGCNA method . Methods Identified differentially expressed miRNA profiles, and miRNAs from clinical related module was obtained by WGCNA method. The overlap of module genes and differentia)ly expressed miRNAs were considered as key miRNAs. Subsequently predicted mRNAs and upstream transcription fac tors were acquired. Then intersected mRNAs of predicted targets and differentially expressed mRNAs were ide n的ed, based on which GO and KEGG enrichment analysis were performed. Finally, regulatory miRNA-mRNA network, taking PP! relationship into consideration, was constructed and analyzed by Cytoscape. Results A total of 6 differentially expressed and clinical related miRNAs were identified as key miRNAs, in which 2 were upregulated and 4 were downregulated. SP4, LHX3, NFIC, MYC and VSX2 were considered as key transcription factors of key miRNAs. GO and KEGG analysis indicated multiple processes and pathways were involved, among which Chemokine signaling pathway and p53 signaling pathway were worthy of attention. Conclusion The expression of miRNA-mRNA in osteoporosis is regulated through multiple ways and multiple targets, and hsa-miR-4500, CDKNlA and MAP2K7 are the core regulatory networks. This study provides a new insight into the study of the molecular mechanism and uncovering of new potential targets of osteoporosis, which has important g u心ng significance for elucidating the pathogenesis of osteoporosis and the development of potential drugs. [ABSTRACT FROM AUTHOR]