Structural characterization and biological evaluation of a new O-acetyl-1,4-linked-β-d-mannan possessed potential application in hydrophilic polymer materials from Dendrobium devonianum.

To explore the active polysaccharides from Dendrobium devonianum , a novel O -acetylmannan (DDP-1) with molecular weight of 117 kDa was isolated from D. devonianum. The chemical and instrumental analysis indicated that the DDP-1 was a homopolysaccharide containing a backbone chain composed of →4)- β - d -Man p -(1 → (71.4%) residue with internal →4)-2- O -acetyl- β - d -Manp-(1 → (14.2%), →4)-3- O -acetyl- β - d -Man p -(1 → (7.1%), and non-reducing end β - d -Man p -(1 → (7.3%) residues. Anticancer assay in vitro revealed that DDP-1 had anticancer activity against the growth of HepG2 and MCF-7 cancer cells. Moreover, cytokine secretion assays also presented that DDP-1 can promote cytokine production of TNF- α and IL-6 in THP-1 macrophage stimulated by PMA. Finally, the effects of isolation and purification on the microstructure of DDP-1 was studied by scanning electron microscope. The morphological features of DDP-1 indicated that DDP-1 hold high potential application in hydrophilic polymer materials. Structural characterization and biological evaluation of an key O -acetyl-1,4-linked- β - d -mannan from Dendrobium devonianum. [Display omitted] • The fine structure of Dendrobium devonianum polysaccharide was firstly elucidated. • Anticancer assay in vitro revealed that DDP-1 had cytotoxic activity against the growth of HepG2 and MCF-7 cancer cells. • DDP-1 presented remarkable immune-enhancing properties in vitro. • The microstructure features of DDP-1 indicated that DDP-1 hold high potential application in hydrophilic polymer materials. [ABSTRACT FROM AUTHOR]