Rapid simultaneous determination of coumarins and organic acids in notopterygium by an isocratic micellar liquid chromatography based on Box-Behnken design.

[Display omitted] • The 5 active components in notopterygium and its processed products were determined by MLC for the first time. • The isocratic chromatographic conditions were optimized through Box-Behnken design. • The established MLC method is simple, efficient, and low-cost. This work has developed an isocratic micellar liquid chromatographic method for simultaneous determination of nodakenin, isoimperatorin, psoralen, ferulic acid, and chlorogenic acid in notopterygium and notopterygium processed products for the first time. Analytes were successfully separated within 15 min. The optimized chromatographic conditions were obtained by Box-Behnken design optimization, and the mobile phase consisted of 0.064 M sodium lauryl sulfate aqueous solution and 8.15% n-pentanol with a pH value of 3.04. The analytes were eluted isocratically through a C18 column at a flow rate of 1 mL/min. The linear ranges, limit of detection, limit of quantitation, recovery, intra-day and inter-day precision of the proposed method were validated according to ICH guidelines. The proposed method was successfully applied to the quantitative the 5 active components in notopterygium and its processed products. It is a simple, efficient, and low-cost analytical method that provides an alternative method for the determination of the 5 active ingredients in notopterygium. [ABSTRACT FROM AUTHOR]