A disposable printed amperometric biosensor for clinical evaluation of creatinine in renal function detection.

In clinical practice, sera creatinine level is regarded as a crucial biomarker for the diagnosis, staging and monitoring of kidney disease. An amperometric biosensor is rapid, accurate, and cost-effective, with a portability and a simple operation. Herein, we report for the firsttime a disposable, printed amperometric biosensor for the clinical evaluation of creatinine in renal function detection. The sensor is constructed based on Prussian blue/carbon-graphite paste as the working electrode and the immobilization of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. The creatinine biosensor shows a linear detection range from 0.05 to 1.4 mM with a detection time of about 3 min. In addition, the sensor shows a high stability that can maintain above 86% of the initial activity after being stored for over 4 months. Moreover, the sensor shows almost the same results as those with the Jaffe method for measuring the real blood samples. We anticipate that the creatinine biosensor could be widely used in the medical and healthcare areas, especially for at-home testing and onsite medical examinations. [Display omitted] • The work reports for the first time the development of a disposable, miniaturized and cost-effective creatinine biosensor. • The biosensor shows an accurate, sensitive and rapid determination of serum creatinine in renal function detection. • The biosensor demonstrates a similar detection performance to the Jeff Method that is used in hospital. • The biosensor exhibits a great potential for the at-home testing and the on-site medical examination. [ABSTRACT FROM AUTHOR]