Preparation of pH‐Sensitive Molecularly Imprinted Polymer via Dual‐Monomer for Selective Solid‐Phase Extraction of Ribavirin from Human Urine and Pharmaceutical Samples.

The specific molecularly imprinted polymer for ribavirin was fabricated by using 3‐allyloxy‐1, 2‐propanediol and acrylic acid as the functional monomers, divinylbenzene and N, N'‐methylenebisacrylamide as the cross‐linkers, and azobisisobutyronitrile as the initiator. It was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy combined with an energy dispersive X‐ray analyzer, and thermogravimetric analysis. Several factors affecting the extraction of ribavirin, such as pH, contact time, and temperature were also investigated. The molecularly imprinted polymer indicated high sorption capacity (3.1 mg g−1) for ribavirin, and the adsorption equilibrium was established after 15 min. The adsorption isotherm results could be well described with the Freundlich isotherm model. The linear range was 0.5–80 mg L−1, and recoveries ranging from 93 % to 102 % were achieved. The detection limit of ribavirin was identical at 0.82 μg L−1, and the relative standard deviation was 0.265 %. It was successfully used for ribavirin analysis in human urine and pharmaceutical samples. [ABSTRACT FROM AUTHOR]