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SERCA interacts with chitin synthase and participates in cuticular chitin biogenesis in Drosophila.
- Source :
-
Insect Biochemistry & Molecular Biology . Jun2022, Vol. 145, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- The biogenesis of chitin, a major structural polysaccharide found in the cuticle and peritrophic matrix, is crucial for insect growth and development. Chitin synthase, a membrane-integral β-glycosyltransferase, has been identified as the core of the chitin biogenesis machinery. However, a yet unknown number of auxiliary proteins appear to assist in chitin biosynthesis, whose precise function remains elusive. Here, we identified a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), in the fruit fly Drosophila melanogaster , as a chitin biogenesis-associated protein. The physical interaction between Dm SERCA and epidermal chitin synthase (Krotzkopf verkehrt, Kkv) was demonstrated and analyzed using split-ubiquitin membrane yeast two-hybrid, bimolecular fluorescent complementation, pull-down, and immunoprecipitation assays. The interaction involves N-terminal regions (aa 48–81 and aa 247–33) and C-terminal regions (aa 743–783 and aa 824–859) of Dm SERCA and two N-terminal regions (aa 121–179 and aa 369–539) of Kkv, all of which are predicted be transmembrane helices. While tissue-specific knock-down of DmSERCA in the epidermis caused larval and pupal lethality, the knock-down of DmSERCA in wings resulted in smaller and crinkled wings, a significant decrease in chitin deposition, and the loss of chitin lamellar structure. Although Dm SERCA is well-known for its role in muscular contraction, this study reveals a novel role in chitin synthesis, contributing to our knowledge on the machinery of chitin biogenesis. [Display omitted] • Epidermal chitin synthase-interacting candidates are identified by TAP-MS in fly. • Sarco/endoplasmic reticulum Ca2+-ATPase interacts with epidermal chitin synthase. • The Dm SERCA-Kkv interaction involves several predicted transmembrane helices. • Dm SERCA functions in cuticle chitin biosynthesis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09651748
- Volume :
- 145
- Database :
- Academic Search Index
- Journal :
- Insect Biochemistry & Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 157121760
- Full Text :
- https://doi.org/10.1016/j.ibmb.2022.103783