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Microarray analysis and PCR validation of genes associated with facultative parthenogenesis in Meleagris gallopavo (Turkey).
- Source :
-
Theriogenology . Jul2022, Vol. 186, p86-94. 9p. - Publication Year :
- 2022
-
Abstract
- A cDNA microarray containing 43,661 differentially expressed genes was carried out on the blastoderm of fertilized and facultative parthenogenic turkey embryos at different hours of development. The total number of up-regulated (UR) and down-regulated (DR) genes at 0, 12, and 24 h of development were 725 and 1436, 942 and 599, and 589 and 1044, respectively. Common genes between 0 and 12 h, 12 and 24 h, and 0 and 24 h were 55, 67, and 110, respectively. The proportion of genes showing above 50-fold UR and DR at 0, 12, and 24 h of development were 2.0% and 1.5%, 0.5% and 1.2%, and 0.2% and 1.1%, respectively. Eight UR genes were validated (APOA1 , THRAP3 , ARL14EP , PSAP , MOG , MYBPC2 , MTIF3 and EDG4) and relative expression of six of them was significantly higher (P ≤ 0.05) in parthenogenic embryos, while two genes showed non-significant (P ≥ 0.05) variation. The expression of BCL11A , PRP4B , TCP1 , and TPI1 genes was significantly (P ≤ 0.05) DR in parthenotes in the micro-array study, while the TCP1 gene was up-regulated, and there was no variation in TPI1 gene expression in the PCR validation study. In conclusion, our findings demonstrate differential expression of a large number of genes in parthenotes at different stages of embryo development compared to fertilized embryos. Up-regulation of APOA1, MYBPC2, TCP1, and THRAP3 genes, suggest their crucial role in spontaneous facultative parthenogenic development in turkey birds. • This is the first microarray report for turkey parthenogenetic embryos. • The development of parthenogenetic embryo is comparatively slower than the normally fertilized embryos. • Five genes were found common among the three different stages of parthenogenesis. • Genes including APOA1, MYBPC2, TCP1, and THRAP3 were important for spontaneous facultative parthenogenic embryo development. [ABSTRACT FROM AUTHOR]
- Subjects :
- *WILD turkey
*TURKEYS
*PARTHENOGENESIS
*GENES
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 0093691X
- Volume :
- 186
- Database :
- Academic Search Index
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 157033239
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2022.03.027