Voltammetric Detection of Inositol Using a Platinum Based Electrode.

An electrochemical detection of inositol content using platinum (Pt)-based noble metal electrode is investigated. In this work, the electrochemical behavior of the platinum electrode has been studied and analyzed using a three-electrode system against a silver–silver chloride (Ag/AgCl) reference electrode and a steel counter electrode. Differential pulse voltammetry technique has been employed for this experimental study. A satisfactory linear range of operation was obtained from 50 to 400 μ M with LOD = 1 9. 2 8 μ M. Electrochemical responses for several inositol concentrations 50, 80, 100, 200, 300 and 400 μ M have also been analyzed using principal component analysis (PCA) with effective data clustering. A good class separability index (SI) was found to be 142.91. In addition, a prediction estimation of inositol contents using partial least square regression (PLSR) and principal component regression (PCR) algorithms were also evaluated and prediction accuracies of 93.69% and 93.71% were obtained, respectively. Moreover, the application of the Pt electrode over real orange juice sample extracts revealed satisfactory recovery rate of 96.18%. Thus, this technique of electrochemical system may be subjected for inositol detection in our daily-life food (especially juice, beverages) consumption. [ABSTRACT FROM AUTHOR]