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A New Generation of Lineage Tracing Dynamically Records Cell Fate Choices.
- Source :
-
International Journal of Molecular Sciences . May2022, Vol. 23 Issue 9, p5021-5021. 11p. - Publication Year :
- 2022
-
Abstract
- Reconstructing the development of lineage relationships and cell fate mapping has been a fundamental problem in biology. Using advanced molecular biology and single-cell RNA sequencing, we have profiled transcriptomes at the single-cell level and mapped cell fates during development. Recently, CRISPR/Cas9 barcode editing for large-scale lineage tracing has been used to reconstruct the pseudotime trajectory of cells and improve lineage tracing accuracy. This review presents the progress of the latest CbLT (CRISPR-based Lineage Tracing) and discusses the current limitations and potential technical pitfalls in their application and other emerging concepts. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FATE mapping (Genetics)
*RNA sequencing
*CRISPRS
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 23
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 156872388
- Full Text :
- https://doi.org/10.3390/ijms23095021