浓缩生长因子干预大鼠骨髓间充质干细胞的增殖、成骨分化及迁移.

BACKGROUND: On the basis of studying the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells, it is one of the hot topics in regenerative medicine to reveal their migration mechanism. OBJECTIVE: To investigate the effects of concentrated growth factor on proliferation, osteogenic differentiation, and migration of rat bone marrow mesenchymal stem cells. METHODS: Concentrated growth factor was extracted from rat heart vein by variable speed centrifugation. Rat bone marrow mesenchymal stem cells were cultured in vitro and divided into experimental and control groups. Cells in the experimental groups were cultured in α-MEM complete medium supplemented with 5%, 10% and 20% concentrated growth factors by volume fraction, separately. Cells in the control group were cultured in α-MEM complete medium without concentrated growth factor. Cell proliferation was detected by CCK-8 assay. Cell migration was detected by scratch test and Transwell migration chamber assay. Osteogenic differentiation was detected by alizarin red staining. RESULTS AND CONCLUSION: (1) Concentrated growth factor with different volume fractions could promote the proliferation of rat bone marrow mesenchymal stem cells with different volume fractions. (2) The scratch test showed that the migration of rat bone marrow mesenchymal stem cells could be promoted in the experimental groups, and the migration rate of 10% concentrated growth factor group was fastest at 12 hours. Transwell migration chamber assay showed that the number of migrated rat bone marrow mesenchymal stem cells in the experimental groups was higher than that in the control group. The number of migrated rat bone marrow mesenchymal stem cells was highest in the 10% concentrated growth factor group. (3) Alizarin red staining showed that the total number of calcium nodules in the experimental groups was higher than that in the control group on day 7. (4) The results showed that all concentrated growth factors with different volume fractions could promote the proliferation, differentiation, and migration of rat bone marrow mesenchymal stem cells in vitro. The 10% concentrated growth factor had a faster ability to promote the migration of rat bone marrow mesenchymal stem cells. [ABSTRACT FROM AUTHOR]