Back to Search
Start Over
Transport of Acrosomal Enzymes by KIFC1 via the Acroframosomal Cytoskeleton during Spermatogenesis in Macrobrachium rosenbergii (Crustacea, Decapoda, Malacostracea).
- Source :
-
Animals (2076-2615) . Apr2022, Vol. 12 Issue 8, pN.PAG-N.PAG. 21p. - Publication Year :
- 2022
-
Abstract
- Finally, a Zeiss laser scanning confocal microscope (LSM880, Carl Zeiss, Oberkochen, Germany) was used to observe the distribution of Mr-KIFC1 and Mr-Acrosin and the colocalization status of Mr-KIFC1 and AFS. At the same time, during the entire process of spermatogenesis in I M. rosenbergii i , Mr-KIFC1 localization was closely linked to nucleus reshaping (Figure 10a3-f3), which may explain the function of Mr-KIFC1 as a motor protein involved in sperm nucleus deformation. The above results indicate that Mr-Acrosin and Mr-KIFC1 are both involved in spermatogenesis and that Mr-Acrosin is transported by Mr-KIFC1, which leads us to speculate that Mr-KIFC1 may transport Mr-Acrosin along the AFS structure to the acrosome to exert protease-like effects and participate in the sperm-egg union in I M. rosenbergii i . 5. We also discovered that microtubules formed an umbrella-shaped AFS structure, and Mr-KIFC1 signals overlapped with tubulin, which may suggest that AFS act as scaffolds for Mr-KIFC1 to transport cargoes that are later organized into functional acrosomes. Multiple Sequence Alignment, Phylogenetic Evolutionary Tree Analysis and Protein Structu... The putative protein sequences of Mr-KIFC1 and Mr-Acrosin were obtained by the online sequence processing toolkit SMS (http://www.bio-soft.net/sms/, accessed on 10 March 2021). [Extracted from the article]
Details
- Language :
- English
- ISSN :
- 20762615
- Volume :
- 12
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Animals (2076-2615)
- Publication Type :
- Academic Journal
- Accession number :
- 156479463
- Full Text :
- https://doi.org/10.3390/ani12080991