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Shared-cathode closed bipolar electrochemiluminescence cloth-based chip for multiplex detection.
- Source :
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Analytica Chimica Acta . May2022, Vol. 1206, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
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Abstract
- Electrochemiluminescence (ECL) chips have been widely used in the field of medical diagnosis. However, most of these chips currently in use are costly and require high amounts of sample. In this work, we present, for the first time, a shared-cathode closed bipolar electrochemiluminescence (SC-CBP-ECL) cloth-based chip, which can be used for multiplex detection. The SC-CBP-ECL chips ($0.03–0.05 for each chip) are manufactured using carbon ink- and wax-based screen-printing techniques, without the need for expensive and complex fabrication equipment. Under optimised conditions, the SC-CBP-ECL chips were successfully used for coinstantaneous detection of glucose in double ECL systems (i.e., Ru(bpy) 3 2+ and luminol), with corresponding linear ranges of 0.05–1 mM and 0.05–10 mM, and detection limits of 0.0382 mM and 0.0422 mM. To our knowledge, this is the first report on the application of fibre material-based closed bipolar electrodes (C-BPE) combined with double ECL systems. Furthermore, the SC-CBP-ECL chips exhibit an acceptable specificity and good reproducibility and stability and can be used for glucose detection in human serum samples with a good agreement compared with the clinical method. Finally, the SC-CBP-ECL chips could be successfully used for simultaneous detection of seven glucose samples and also show potential for simultaneous detection of three different targets (hydrogen peroxide [H 2 O 2 ], glucose, and uric acid [UA]). Therefore, we believe that the chip described in this study has broad potential application in the field of cost-effective multiplex detection. [Display omitted] • A shared-cathode closed bipolar electrochemiluminescence (SC-CBP-ECL) is reported. • The combination of cloth-based C-BPE and double ECL system is presented. • The SC-CBP-ECL can be used for coinstantaneous detection of seven glucose samples. • The SC-CBP-ECL can be applied for simultaneous detection of three different targets. • The proposed multiplex detection method is cost-effective. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032670
- Volume :
- 1206
- Database :
- Academic Search Index
- Journal :
- Analytica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 156469386
- Full Text :
- https://doi.org/10.1016/j.aca.2022.339446