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Antibacterial effect and mechanism against Escherichia coli of polysaccharides from Armillariella tabescens mycelia.
- Source :
-
International Journal of Biological Macromolecules . May2022, Vol. 207, p750-759. 10p. - Publication Year :
- 2022
-
Abstract
- The objectives of this study were (1) to prepare Armillariella tabescens mycelia polysaccharides (PAT) with remarkably growth inhibitory effect on typical food-borne pathogenic bacteria using a green and efficient polyamide method and (2) to explore the antibacterial mechanism of PAT for use as a natural antibacterial agent. The sugar and uronic acid contents of PAT were 93.41% and 12.24%, respectively. PAT could inhibit the growth of Escherichia coli , Proteus vulgaris , Bacillus subtilis , and Staphylococcus aureus cells, with minimum inhibitory concentrations of 0.5, 1.0, 4.0, and 4.0 mg/mL, respectively. Ultra-high-resolution field emission scanning electron microscopy and high-resolution transmission electron microscopy analysis revealed cell wall and membrane rupture of E. coli treated with PAT. Further, 0.5–4.0 mg/mL PAT was found to significantly (P < 0.01) and concentration-dependently increase the conductivity of the broth, exudation of the intracellular protein, and alkaline phosphatase and β-galactosidase activities. Confocal laser scanning microscopy revealed morphological changes in E. coli DNA after PAT treatment and intracellular reactive oxygen species accumulation; flow cytometry revealed E. coli cell apoptosis. Our findings provide a theoretical basis and technical support for the development of PAT as a natural antibacterial product. • Preparation of antibacterial polysaccharides (PAT) using the polyamide method • PAT damaged E. coli cell wall and cell membrane. • PAT induced DNA morphological changes in E. coli. • PAT caused intracellular ROS accumulation in E. coli. • Multi-targeting ability of PAT, inducing drug resistance was difficult for E. coli. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 207
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 156451642
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2022.03.086