通络益气方对博来霉素致大鼠肺纤维化的治疗作用研究.

To study the effect of Tongluo Yiqi Recipe on lung tissue pathology and lung tissue hydroxyproline (HYP) in a rat model induced by bleomycin. 36 SPF-grade healthy 6-week-old Wistar male rats were placed under SPF-grade conditions. After 3 days of adaptive trial rearing, they were divided into 3 groups: blank group, model group, and treatment group, with 12 rats in each group. Except for the blank group, the other two groups were awake and placed in a 30 cm×30 cm×20 cm self-made glass box connected to the small animal nebulization dosing device. The inhalation concentration was 5 g/L (50%). Lymycin, sprayed for 20 minutes, rested for 5 minutes, stimulated continuously for 2 hours a day, stimulated continuously for 4 weeks. The blank group inhaled 0.9% saline under the same conditions, and the operation method was the same as that of the other two groups. The rats in the treatment group were given Tongluo Yiqi Recipe from the 2nd day after modeling, and the Tongluo Yiqi Recipe solution was given to the rats by gavage of 1 mL/100 g of liquid medicine according to their body weight, each time 1 hour before nebulization; Both the model group and the normal group were given saline (1 mL/100 g) by gavage. After 4 weeks of continuous stimulation, rats in each group were anesthetized with 1% sodium pentobarbital (50 mg/kg), and after their muscles were relaxed and breathing was stable, they were fixed on their backs on a special board, and the lungs were scanned by animal CT. After the last induction, rats in each group were anesthetized by intraperitoneal injection of 1% sodium pentobarbital within 24 hours, and then lung tissues were taken for HE and Masson staining to observe the pathological HE staining, Masson staining and the content of HYP in the lung tissues of rats in each group. Compared with the blank group, the HYP of the treatment group and the model group were increased (P<0.05); compared with the model group, the HYP of the treatment group was significantly lower (P<0.05). Tongluo Yiqi Decoction can reduce the deposition of collagen in lung tissues of pulmonary fibrosis rats, and play a role in treating pulmonary fibrosis, which is related to the inhibition of HYP collagen expression by Tongluo Yiqi Decoction. [ABSTRACT FROM AUTHOR]