菲牛蛭粗提物冻干粉保护剂的优化.

19 (34) orthogonal test was used to investigate the protective effect of trehalose, PEG2000 and mannitol on freeze — dried powder of Haudinaria manillensis crude extract wider different time and temperature conditions. The results showed that among the three protective agents, PEG2000 had the strongest protective effect and trehalose was the weakest. When the average activity within 24 h was used as the index, the optimal mass fraction combination of trehalose, PEG2000 and mannitol was 4%, 4% mid 2%. Taking the average activity after 15 d at ( —18 ± 2 ) ºC and the average activity after 15 d at (27 ± 3 ) °C as the indexes, the optimal mass fraction combi-nation of those agents was 2%, 4% and 2%. [ABSTRACT FROM AUTHOR]