4 种黄精属植物的基因组大小比较分析.

In order to enrich the data on genome sizes of Polygonatum species, flow cytometry was used for ploidy detection and estimation of genome sizes of four Polygonatum species recorded in the Chinese Pharmacopoeia, including P. kingianum, P. sibiricum, P. cyrtonema, and P. odoratum from different habitats. The results show that Phyllostachys pubescens can be used as a suitable reference standard for the estimation of genome sizes of Polygonatum species. Regarding the four diploid Polygonatum species, the present study found that the genome size of P. odoratum（13 829-14 083 Mb） is similar to that of P. kingianum（13 780 Mb); the genome size of P. sibiricum（12 792-13 516 Mb） is slightly smaller than that of P. odoratum and P. kingianum; and the genome size of P. cyrtonema（9 311 Mb） is the smallest. The genome size of triploid P. cyrtonema is 11 032-11 267 Mb, which is 1.2 times larger than that of diploid P. cyrtonema. The genome size of tetraploid P. kingianum is 12 088-12 411 Mb, which is 89% of the size of diploid P. kingianum. The results append the data on the genome sizes of Polygonatum species, which will provide a reference for a follow-up study on genomics, species classification, and population evolution. [ABSTRACT FROM AUTHOR]