Production of fucoxanthin from the microalga Tisochrysis lutea in the bubble column photobioreactor applying mass transfer coefficient.

Fucoxanthin is one of the most vital pigments during photosynthesis and is extracted from golden-brown micro-algae such as Tisochrysis lutea. The present study investigates the constant volumetric mass transfer coefficient (k L a), for the first time as the scale-up strategy to change the scale from 500 mL to 2-L cultivation flasks, and 5-L bubble column photobioreactor for fucoxanthin production in T. lutea. The cell density and fucoxanthin content were improved because of through fine aeration, nutrients, and light availability by successful laboratory scale up. Fucoxanthin productivity obtained 21.20, 22.99, and 24.96 mg L–1day–1 for 500 mL, 2-L bottle, and 5-L bubble column photobioreactor, respectively. In addition, the biomass productivity enhanced from 267.5 to 275 and 284 mg L–1day–1 in three mentioned scales, respectively. Eventually, the scale up process for the production of fucoxanthin was succeeded from 500 mL bottle to 5-L photobioreactor using constant (k L a) under laboratory conditions. • Fucoxanthin is one of the most important carotenoids with several health benefits. • The production of this valuable metabolite from Tisochrysis lutea was investigated in a bubble column photobioreactor. • The constant volumetric mass transfer coefficient, (kLa) used as the scale-up strategy for the production of fucoxanthin from 500 mL to 5-L bubble column photobioreactor. • Fucoxanthin productivity was enhanced up to 24.96 mg L–1 day–1 in f/2 enriched medium by starch. [ABSTRACT FROM AUTHOR]