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Bulk segregation analysis in the NGS era: a review of its teenage years.

Bulk segregation analysis in the NGS era: a review of its teenage years.

Authors :
Li, Zhiqiang
Xu, Yuhui
Source :
Plant Journal. Mar2022, Vol. 109 Issue 6, p1355-1374. 20p.
Publication Year :
2022

Abstract

SUMMARY Bulk segregation analysis (BSA) utilizes a strategy of pooling individuals with extreme phenotypes to conduct economical and rapidly linked marker screening or quantitative trait locus (QTL) mapping. With the development of next‐generation sequencing (NGS) technology in the past 10 years, BSA methods and technical systems have been gradually developed and improved. At the same time, the ever‐decreasing costs of sequencing accelerate NGS‐based BSA application in different species, including eukaryotic yeast, grain crops, economic crops, horticultural crops, trees, aquatic animals, and insects. This paper provides a landscape of BSA methods and reviews the BSA development process in the past decade, including the sequencing method for BSA, different populations, different mapping algorithms, associated region threshold determination, and factors affecting BSA mapping. Finally, we summarize related strategies in QTL fine mapping combining BSA. Significance Statement: This paper provides a landscape of BSA methods in the next‐generation sequencing (NGS) era and a guideline to select a proper method for bulk segregation analysis mapping design. In addition, quantitative trait locus fine mapping strategies are summarized for researchers in the field. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09607412
Volume :
109
Issue :
6
Database :
Academic Search Index
Journal :
Plant Journal
Publication Type :
Academic Journal
Accession number :
156056855
Full Text :
https://doi.org/10.1111/tpj.15646