Phosphoproteomics profiling reveals a kinase network conferring acute myeloid leukaemia intrinsic chemoresistance and indicates HMGA1 phosphorylation as a potential influencer.

(J) ChIP-qPCR assay in 293T cells that ectopically overexpress Flag-tagged HMGA1-WT or HMGA1-S3A, and with siSP1 in these Flag-HMGA1 expressing cells. (B) Western blotting for pan-phospho-serine levels in 293T cells transduced with MOCK, Flag-tagged HMGA1-WT, HMGA1-S99A, HMGA1-S102A, HMGA1-S103A or HMGA1-S3A. ChIP analysis using anti-flag antibodies or control normal mouse IgG was performed in four groups: HMGA1-WT/siCtrl, HMGA1-WT/siSP1, HMGA1-S3A/siCtrl and HMGA1-S3A/siSP1. [Extracted from the article]