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The Hob proteins are novel and conserved lipid-binding proteins at ER--PM contact sites.
- Source :
-
Journal of Cell Science . Mar2022, Vol. 135 Issue 5, p1-11. 11p. - Publication Year :
- 2022
-
Abstract
- Membrane contact sites are critical junctures for organelle signaling and communication. Endoplasmic reticulum-plasma membrane (ER-PM) contact sites were the first membrane contact sites to be described; however, the protein composition and molecular function of these sites is still emerging. Here, we leverage yeast and Drosophila model systems to uncover a novel role for the Hobbit (Hob) proteins at ER-PM contact sites. We find that Hobbit localizes to ER-PM contact sites in both yeast cells and the Drosophila larval salivary glands, and this localization is mediated by an N-terminal ER membrane anchor and conserved C-terminal sequences. The C-terminus of Hobbit binds to plasma membrane phosphatidylinositols, and the distribution of these lipids is altered in hobbit mutant cells. Notably, the Hobbit protein is essential for viability in Drosophila, providing one of the first examples of a membrane contact site-localized lipid binding protein that is required for development. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CARRIER proteins
*SALIVARY glands
*PROTEINS
*CELL membranes
*DROSOPHILA
*LIPIDS
Subjects
Details
- Language :
- English
- ISSN :
- 00219533
- Volume :
- 135
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Cell Science
- Publication Type :
- Academic Journal
- Accession number :
- 155919953
- Full Text :
- https://doi.org/10.1242/jcs.259086